SPECIALIZED EQUIPMENT

 

Modern animal facility - operating in SPF standard; houses several strains of knockout and transgenic mice and rats, kept in individually ventilated cages (IVC). The facility is equipped in:

  • PeriCam PSI System (Perimed) - Laser Speckle Contrast Analysis (LASCA) for the assessment of microcirculation
  • Vevo 2100 (VisualSonics) - ultrasound system for high-resolution imaging of small animals;
  • IVIS© Lumina II (Caliper Life Science) - imaging system for both fluorescent and bioluminescent imaging in vivo;
  • Exer 3/6 (Columbus Instruments) - animal treadmill for mice and rats;
  • Aurora1300A: 3-in-1 Whole Animal System (Aurora Scientific) – system for the measurement of rodent muscle properties in situ, in vivo and in vitro;
  • Grip Strength Meter (Ugo Basile) - a system for determining the gripping strength (the maximum force) of small laboratory animals (rat, mouse);
  • scil Vet abc – hematology analyzer (different animal species can be analyzed);
  • SPOTCHEM EZ SP-4430 (Arkray) - automated analyzer for clinical chemistry;
  • IBIDI Pump system -organ-on-a-chip equipment.

 

Flow cytometry laboratory equipped in:

  • BD LSRFortessa (BD Biosciences) - flow cytometer;
  • MoFloTM XDP (Beckman Coulter) - high-speed cell sorter;
  • ImageStreamX (Amnis Corporation) - system which integrates the features of flow cytometry and fluorescent microscopy combined with a modern system of image analysis.

 

Histological laboratory equipped in:

  • Automated tissue processor;
  • Automated tissue embedding system;
  • Microtome - with the Section-Transfer-System;
  • Cryostat;
  • Automated staining system for paraffin sections;
  • Laser Microdissection LMD7000 (Leica).

 

Other equipment:

  • Chambers for hypoxic conditions/hypoxic incubators/hypoxic laminar for cells culturing in low oxygen concentration;
  • Bioanalyzer 2100 (Agilent) for checking the RNA integrity;
  • Real-time PCR StepOnePlus (Applied Biosystem) for analysis of a gene expression profile on mRNA level, as well as microRNAs analysis;
  • AmpliSpeed slide cycler (Beckman Coulter) for RT-PCR reaction from very small amount of samples (even one cell);
  • FlexMap3D (Luminex) for measurement of a dozen or so of cytokines/growth factors in a small volume of sample (serum/plasma/medium);
  • MUSE MultiElectrode Arrays - measurement of cardiomyocytes electrophysiological activity.