Research topics

  • Stem cell biology - mechanisms of cellular reprogramming and differentiation:
  1. Mechanisms of differentiation of induced pluripotent stem cells into cardiomyocytes, endothelial cells and skeletal muscle cells
  2. Satellite cells differentiation
  3. Hematopoietic stem cell differentiation;
  • Molecular mechanisms of Duchenne muscular dystrophy - regeneration and vascularisation of skeletal and cardiac muscles;
  • Vascular biology: molecular mechanisms of angiogenesis and function of endothelial cells;
  • Mechanisms of myocardial damage and regeneration;
  • Medical biotechnology: gene and cell therapy in modulation of neovascularization, heart and muscle regeneration;
  • Cancer biology and anti-cancer therapy: mechanisms of tumor initiation, growth, metastasis and resistance to therapy;
  • Role of hypoxia, antioxidant genes and microRNAs in regulation of gene expression;
  • The role of heme oxygenase-1 (HO-1), Nrf2 and microRNAs in cardiovascular system, skeletal muscles, kidney and cancers.


Selected techniques


  • Flow cytometry & cell sorting;
  • Molecular in vivo imaging;
  • Laser microdissection method;
  • Gene editing by CRISPR/Cas;
  • siRNA, shRNA & microRNAs technology;
  • Angiogenic assays in vitro: tube formation on Matrigel, aortic ring assay, spheroid assay;
  • In vivo models: hind limb ischemia, wound healing, tumor inoculation, chemical carcinogenesis, mouse model of myocardial infarction;
  • Induced pluripotent stem cells (iPSCs) technology;
  • Gene cloning, construction of plasmids and viral vectors: retroviral, lentiviral, adenoviral, AAV;
  • Regulated gene expression: hypoxia-induced and tetracycline-induced expression systems, establishing of stably-transfected cell lines;
  • Isolation and culture of primary cells: satellite cells, hematopoietic stem cells, mesenchymal stromal cells, endothelial cells, renal proximal epithelial cells.


Animal models, kept in modern animal facility (SPF - specific pathogen free)


  • miR-146a knockout mice
  • miR-378a knockout mice
  • mdx mice (dystrophin knockout)
  • HO-1 knockout mice
  • HO-1 knockout GFP mice
  • HO-1 floxed mice (knockout and knockin)
  • Nrf2 transcriptional knockout mice
  • hyperglycaemic mice (db/db)
  • Cre-recombinase expressing mice (Cdh5-Cre, Cdh5-CreER, Hoxb5-CreER)
  • NSG-SGM3 mice
  • Hoxb5-mCherry mice
  • miR-34a floxed mice
  • Neo1 floxed mice
  • Nrf2 floxed mice